Cloning and expression of the Thiobacillus ferrooxidans glutamine synthetase gene in Escherichia coli
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چکیده
منابع مشابه
Cloning and expression of the Thiobacillus ferrooxidans glutamine synthetase gene in Escherichia coli.
The glutamine synthetase (GS) gene glnA of Thiobacillus ferrooxidans was cloned on recombinant plasmid pMEB100 which enabled Escherichia coli glnA deletion mutants to utilize (NH4)2SO4 as the sole source of nitrogen. High levels of GS-specific activity were obtained in the E. coli glnA deletion mutants containing the T. ferrooxidans GS gene. The cloned T. ferrooxidans DNA fragment containing th...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1985
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.164.3.1386-1389.1985